p iκb α Search Results


phosphorylated p iκb α  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology phosphorylated p iκb α
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Phosphorylated P Iκb α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb+%CE%B1/pm26865401-30-8-33?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
phosphorylated p iκb α - by Bioz Stars, 2026-08
96/100 stars
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nfkbia(ser32/36) polyclonal antibody  (Bioss)
94
Bioss nfkbia(ser32/36) polyclonal antibody
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Nfkbia(ser32/36) Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb+%CE%B1/custom%40bs-2513r%4035625074?v=Bioss
Average 94 stars, based on 1 article reviews
nfkbia(ser32/36) polyclonal antibody - by Bioz Stars, 2026-08
94/100 stars
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rabbit polyclonal antibodies against phosphorylated iκb-α (p-iκb-α  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company rabbit polyclonal antibodies against phosphorylated iκb-α (p-iκb-α
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Rabbit Polyclonal Antibodies Against Phosphorylated Iκb α (P Iκb α, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb+%CE%B1/pm26256698-40-7-26?v=ImmunoWay+Biotechnology+Company
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies against phosphorylated iκb-α (p-iκb-α - by Bioz Stars, 2026-08
90/100 stars
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iκbα  (Abmart Inc)
86
Abmart Inc iκbα
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Iκbα, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb+%CE%B1/pmc12788684-134-57-58?v=Abmart+Inc
Average 86 stars, based on 1 article reviews
iκbα - by Bioz Stars, 2026-08
86/100 stars
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p iκb α  (Huabio Inc)
86
Huabio Inc p iκb α
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
P Iκb α, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb+%CE%B1/pm39461197-163-33-36?v=Huabio+Inc
Average 86 stars, based on 1 article reviews
p iκb α - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of IκB-α was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.

Journal: International journal of molecular medicine

Article Title: Ginsenoside Rg1 exerts a protective effect against Aβ₂₅₋₃₅-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway.

doi: 10.3892/ijmm.2016.2485

Figure Lengend Snippet: Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of IκB-α was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.

Article Snippet: Primary antibodies against NF-κB (p65) (sc-372), IκB-α (sc-371), phosphorylated (p-)IκB-α (sc-8404), lamin B (sc-6217), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-47724), inducible nitric oxide synthase (iNOS) (sc-651), Bcl-2 (sc-7382), Bax (sc-7480) were all purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Activation Assay, Cell Culture, Translocation Assay, Protein Extraction, Expressing, Western Blot, Phospho-proteomics, Standard Deviation, Control